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Resistance can be read if:
  1. There is 103cfu/ml in the large section.
  2. The density of bacteria in the small antibiotic section is approximately the same as that in the large section.
  3. If there is no growth or 10 times less bacteria in the small antibiotic sections then the bacterium is considered susceptible / sensitive to the antibiotic in question.

The five smaller sections of the Flexicult test each contain a different antibiotic

  1. *Cephalothin (16 mg/L)
  2. Ciprofloxacin (0.75 mg/L)
  3. Amoxicillin/Clavulanate (16/8 mg/L)
  4. Nitrofurantoin (64 mg/L) (Darker colour)
  5. Trimethoprim (3 mg/L)

The sections are numbered on the bottom of the test

(*Cephalothin is used to predict susceptibility to other first generation Cephalosporin such as cefalexin)

After overnight incubation, bacteria will grow in the antibiotic compartments if the bacteria are resistant (R) to the antibiotic in question. Antibiotic sensitive (or susceptible) (S) bacteria are inhibited by the antibiotic concentration and so will not grow in the relevant bacterial compartment, i.e. they will not become colonies.